photoacoustic signals  (Mini-Circuits)

Journal: Neurophotonics

Article Title: Inducing and monitoring photothrombotic stroke in anesthetic neuroprotection-free mice using functional photoacoustic microscopy

doi: 10.1117/1.NPh.13.2.025007

Figure Lengend Snippet: Experimental design and system setup. (a) Schematic design of the custom-built dual-wavelength OR-PAM system, illustrating optical and acoustic pathways. HWP, half-wave plate; PBS, polarizing beam splitter; CP, coupler; PM-SMF, polarization-maintaining single-mode fiber; BPF, band-pass filter; M, mirror; NDF, neutral density filter; DM, dichroic mirror; L, achromatic lens; OAC, optical/acoustic beam combiner; AL, acoustic lens; WT, water tank; UT, ultrasound transducer; AM, amplifier; DAQ, data acquisition unit; PC, personal computer; SP, scanning pathway. (b) Timeline of research including mouse grouping, skull-thinning, head-fixation training, PT induction, PAM imaging, behavioral tests, and pathological examination. (c) Schematic diagram of the head-restrained awake PAM setup. PA probe, photoacoustic probe. (d) Illustration of targeted PT induced in a single distal MCA branch using 532-nm laser illumination guided by PAM. Brain region abbreviations: M1/M2, primary/secondary motor cortex; S1/S2, primary/secondary somatosensory cortex; V1, visual cortex; AU, auditory cortex; RS, retrosplenial area; BC, barrel field; FL and HL, forelimb and hindlimb regions of the primary somatosensory area. The mouse brain atlas image is adapted from Ref. .

Article Snippet: The photoacoustic signals were amplified by 48 dB using a pair of amplifiers (ZFL-500LN, Mini-Circuits, Brooklyn, New York, United States) and digitized at 500 MHz via a data acquisition card (ATS9371, Alazar Technologies, Quebec, Canada).

Techniques: Imaging

Journal: Biomedical Optics Express

Article Title: Enhancing the pathological diagnosis of oral cancers using broadband ultraviolet photoacoustic microscopy

doi: 10.1364/BOE.560483

Figure Lengend Snippet: Schematic representation of the bUV-PAM system integrating ultraviolet photoacoustic excitation and broadband optical evanescent wave sensing, enabling simultaneous obtaining of photoacoustic morphological images and performing photoacoustic spectral analysis. L: Lens; M: Mirror; OL: Objective lens; P: Polarizer; HWP: Half-wave plate; QWP: Quarter-wave plate; BS: Beam splitter; BPD: Balanced photodiode detector; X–Y stage: Two-dimensional scanning platform; PC: Personal computer; UV pulsed laser: Ultraviolet pulsed laser.

Article Snippet: Following a balanced photodetector (BPD), the output photoacoustic signal was amplified via a low-noise amplifier (ZHL-1-2W-S+, Mini-Circuits, 5–500 MHz) and recorded via a digital acquisition card (ATS9870, Alazar Technologies, 450 MHz bandwidth, 1 GS/s sampling rate).

Techniques:

Journal: Biomedical Optics Express

Article Title: Enhancing the pathological diagnosis of oral cancers using broadband ultraviolet photoacoustic microscopy

doi: 10.1364/BOE.560483

Figure Lengend Snippet: PAMI of normal and cancerous mouse tongue sections. (a)(b) Photoacoustic images and corresponding H&E staining images of normal tongue sections. (c)(d) Magnified regions indicated by the solid and dot-dash blue boxes in (a) and (b). Blue dashed lines separate the tongue mucosa epithelial layer (above) and the tongue mucosa lamina propria (below). Arrow 1 denotes filiform papillae, and arrow 2 points to regular tongue muscle fibers. (e)(f) Virtual staining photoacoustic images and corresponding H&E staining images of OSCC tissue sections. The red dashed line marks the boundary between normal tongue mucosa tissue (below) and OSCC tissue (above). (g)(h) Close-up views of the solid and dot-dash red boxes in (e) and (f). Arrow 3 indicates the absence of filiform papillae, while arrow 4 shows the disruption of the tongue muscle fibers. (i) The nuclear-to-cytoplasmic ratio observed in H&E staining images and photoacoustic images of normal tongue mucosa tissue and OSCC. SD: standard deviation.

Article Snippet: Following a balanced photodetector (BPD), the output photoacoustic signal was amplified via a low-noise amplifier (ZHL-1-2W-S+, Mini-Circuits, 5–500 MHz) and recorded via a digital acquisition card (ATS9870, Alazar Technologies, 450 MHz bandwidth, 1 GS/s sampling rate).

Techniques: Staining, Disruption, Standard Deviation

Journal: Biomedical Optics Express

Article Title: Enhancing the pathological diagnosis of oral cancers using broadband ultraviolet photoacoustic microscopy

doi: 10.1364/BOE.560483

Figure Lengend Snippet: PAMI of normal parotid gland and ACC tissue sections in mice. (a)(b) Photoacoustic and corresponding H&E staining images of normal parotid gland tissue sections. (c)(d) Magnified views of the blue dash and dot-dush boxes in (a) and (b), respectively. Arrow 1 highlights the normal glandular lobule, while arrow 2 indicates the salivary duct. (e)(f) Photoacoustic and corresponding H&E staining images of ACC tissue sections. (g)(h) Enlarged views of the red dash and dot-dush boxes in (e) and (f), respectively. Arrow 3 points to cancer cell clusters, where normal acinar lobules and glandular ducts are absent. Arrow 4 points to localized central necrosis within the cancer cell clusters. (i) The nuclear-to-cytoplasmic ratio observed in H&E staining images and photoacoustic images of normal salivary gland tissue and ACC. SD: standard deviation.

Article Snippet: Following a balanced photodetector (BPD), the output photoacoustic signal was amplified via a low-noise amplifier (ZHL-1-2W-S+, Mini-Circuits, 5–500 MHz) and recorded via a digital acquisition card (ATS9870, Alazar Technologies, 450 MHz bandwidth, 1 GS/s sampling rate).

Techniques: Staining, Standard Deviation

Journal: Biomedical Optics Express

Article Title: Enhancing the pathological diagnosis of oral cancers using broadband ultraviolet photoacoustic microscopy

doi: 10.1364/BOE.560483

Figure Lengend Snippet: PASA of normal mouse mucosa and OSCC tissue sections. (a) Schematic representation of the structural differences between normal oral mucosa and OSCC. E: Epithelium; B: Basal layer; S: Spinous layer; G: Granular layer; K: Keratin layer; BM: Basement membrane; LP: Lamina propria. (b) The average photoacoustic spectral distribution curve of normal oral mucosa and OSCC. (c) Statistical results of the -6 dB bandwidth of normal oral mucosa and OSCC tissue sections. SD: standard deviation.

Article Snippet: Following a balanced photodetector (BPD), the output photoacoustic signal was amplified via a low-noise amplifier (ZHL-1-2W-S+, Mini-Circuits, 5–500 MHz) and recorded via a digital acquisition card (ATS9870, Alazar Technologies, 450 MHz bandwidth, 1 GS/s sampling rate).

Techniques: Membrane, Standard Deviation

Journal: Biomedical Optics Express

Article Title: Enhancing the pathological diagnosis of oral cancers using broadband ultraviolet photoacoustic microscopy

doi: 10.1364/BOE.560483

Figure Lengend Snippet: PASA of normal parotid and ACC tissue sections of mice. (a) Schematic representation of the structure of normal parotid gland and ACC. A: Acinus; I: Intercalated duct; S: Striated duct; E: Excretory duct; MEC: Myoepithelial cell. (b) The average photoacoustic spectral distribution curve of the normal parotid gland and ACC. (c) Statistical results for the -6 dB bandwidth of normal parotid gland and ACC tissue sections. SD: standard deviation.

Article Snippet: Following a balanced photodetector (BPD), the output photoacoustic signal was amplified via a low-noise amplifier (ZHL-1-2W-S+, Mini-Circuits, 5–500 MHz) and recorded via a digital acquisition card (ATS9870, Alazar Technologies, 450 MHz bandwidth, 1 GS/s sampling rate).

Techniques: Standard Deviation

Journal: Biomedical Optics Express

Article Title: Enhancing the pathological diagnosis of oral cancers using broadband ultraviolet photoacoustic microscopy

doi: 10.1364/BOE.560483

Figure Lengend Snippet: PASA distinguishes between different stages of OSCC. (a)–(f) IHC staining for the Ki67 biomarker in oral mucosa sections with various progressive stages. (g) Average photoacoustic spectral distribution curves of the six stages. (h) Statistical results of photoacoustic spectral characteristics at -6 dB bandwidth across stages from normal buccal mucosa to OSCC. CG: Control group; EG: Experimental Group; EG1: Edema injury phase; EG2: Mild dysplasia; EG3: Moderate dysplasia; EG4: Carcinoma in situ and early invasive carcinoma; EG5: Invasive carcinoma. (▴ represents a comparison with other groups, and P < 0.001). SD: standard deviation.

Article Snippet: Following a balanced photodetector (BPD), the output photoacoustic signal was amplified via a low-noise amplifier (ZHL-1-2W-S+, Mini-Circuits, 5–500 MHz) and recorded via a digital acquisition card (ATS9870, Alazar Technologies, 450 MHz bandwidth, 1 GS/s sampling rate).

Techniques: Immunohistochemistry, Biomarker Discovery, Control, In Situ, Comparison, Standard Deviation

Journal: Biomedical Optics Express

Article Title: Enhancing the pathological diagnosis of oral cancers using broadband ultraviolet photoacoustic microscopy

doi: 10.1364/BOE.560483

Figure Lengend Snippet: PASA distinguishes between OSCC and ACC. (a)–(d) IHC staining using different antibodies for diagnosing OSCC and ACC. (e) The average photoacoustic spectral distribution curve of OSCC and ACC. (f) Statistical comparison of -6 dB bandwidth in OSCC and ACC tissues. SD: standard deviation.

Article Snippet: Following a balanced photodetector (BPD), the output photoacoustic signal was amplified via a low-noise amplifier (ZHL-1-2W-S+, Mini-Circuits, 5–500 MHz) and recorded via a digital acquisition card (ATS9870, Alazar Technologies, 450 MHz bandwidth, 1 GS/s sampling rate).

Techniques: Immunohistochemistry, Comparison, Standard Deviation